Glucoamyl L-561

Glucoamylase enzyme for use in saccharification and fermentation

Description

Glucoamyl L-561 is a purified native glucoamylase that functions by the hydrolysis of terminal 1, 4 linked alpha-D-glucose residues from non-reducing ends of polysaccharide chains with release of beta-D-glucose. Its application is to yield monomeric glucose molecules that can be fermented by yeast into biofuels of interest such as ethanol.

Typical Characteristics

  • ‍Activity: 188,000–190,000 u/mL (minimum)
  • Appearance: Brown liquid
  • pH: 3.5–5.0
  • Bulk Density: 1.11–1.16 g/mL
  • Unit Definition: One unit of glucoamylase (u) is the amount of enzyme which hydrolyzes soluble starch to liberate 1 milligram of glucose per hour under the conditions of the assay.

Performance Benefits

  • ‍Glucoamyl L-561 provides the following benefits to ethanol producers.
  • Robust and consistent performance under varied conditions
  • Enzyme conversion kinetics match more closely to yeast growth curve
  • Increased alcohol concentrations
  • Lower residual sugars at end of fermentation
  • Reduced glycerol concentrations throughout fermentation

Application Recommendations

Glucoamyl L-561 is used to saccharify liquefied mashes of whole ground corn. The resultant glucose is fermented by yeast to produce ethanol. Glucoamyl L-561 has a broad operating range for enhanced process flexibility, and performs well within the temperature, and pH ranges typical in whole ground corn batch fermentations. In high gravity fermentations, performance can be further enhanced by the addition of SZM XT-20+ Fungal Alpha Amylase and/or SZM AP-1 Acid Protease.

Dosage Recommendation

Glucoamyl L-561 should be dosed at 0.044–0.059% weight enzyme/weight as is corn. The actual dose required will depend upon the conditions of your fermentation: time, initial pH and the level of solids.

Regulatory Status

  • ‍ISO 9001 registered company
  • Kosher certified
  • GRAS (Generally Recognized as Safe)

GM Status

This product is not a GMO. The enzyme product is manufactured by fermentation of microorganisms that are not present in the final product. The production organisms and the enzyme effectiveness are improved by means of modern technology.

Packaging

Glucoamyl L-561 is available in totes or bulk tankers.

Storage

Glucoamyl L-561 will meet the declared activity upon arrival at your plant. Glucoamyl L-561 can be safely stored in sealed containers. Enzymes should be stored below 77°F (25°C) and sheltered from direct sunlight for maximum stability.

Shelf Life

The enzymatic activity of Glucoamyl L-561 remains at ≥ 95% for six months when stored at 77°F (25°C). The recommended dosage may need to be increased if stored for longer than six months.

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